THE COLORIMETRIC DETERMINATION OF URINARY LIPASE

The colorimetric determination of urinary lipase.

With the use of titrimetric (1)) manometric (2)) stalagmometric (3), and calorimetric (4, 5) techniques, the esterase and lipase activities of sera and many tissues have been measured by employing a wide variety of substrates (6). The vast amount of data that resulted from these studies contains numerous discrepancies in the behavior of these esterolytic enzymes, which are still unexplained. Tw...

The Colorimetric Determination of Urinary Estrin

Kober (1931) was the first to suggest a calorimetric test for urinary estrin. He compared the degree of red color developed after treatment with phenolsulfonic acid in a test sample with a standard cresol red solution. Cohen and Marrian (1934) modified the Kober procedure and determined the hormone content of estrone and estriol fractions by comparison with a standard curve from a set of determ...

The colorimetric determination of lipase and esterase in human serum.

Colorimetric determination and distribution of urinary creatinine and creatine.

“Apparent” creatinine and “total” creatinine levels in urine can be estimated to within 2 per cent accuracy by means of the Jaffe colorimetric reaction, provided that certain requirements for the colorimetric reaction itself are met, and provided that appropriate conditions for the conversion of creatine to creatinine are used. The amount of noncreatinine Jaffe-positive material appears to be n...

The Colorimetric Determination of Glucose.

Several new descriptions of colorimetric methods for the determination of blood sugar have been described in recent years (for example, Polis and Sortwell, 1946; Somogyi, 1945; Nelson, 1944; Haslewood and Strookman, 1939). These procedures are all based on the use of alkaline copper reagents and on the production of molybdenum blue by the action of cuprous copper on complex molybdic acid soluti...